High-Potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa Azurins
journal contributionposted on 16.02.2009, 00:00 by Kyle M. Lancaster, Keiko Yokoyama, John H. Richards, Jay R. Winkler, Harry B. Gray
Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated CuII/I reduction potentials relative to the CuII aquo ion. The high potential and low axial hyperfine splitting (CuII electron paramagnetic resonance A∥) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers.