posted on 2022-02-03, 18:35authored byVasyl Yatsyna, Ali H. Abikhodr, Ahmed Ben Faleh, Stephan Warnke, Thomas R. Rizzo
Coupling vibrational
ion spectroscopy with high-resolution ion
mobility separation offers a promising approach for detailed analysis
of biomolecules in the gas phase. Improvements in the ion mobility
technology have made it possible to separate isomers with minor structural
differences, and their interrogation with a tunable infrared laser
provides vibrational fingerprints for unambiguous database-enabled
identification. Nevertheless, wide analytical application of this
technique requires high-throughput approaches for acquisition of vibrational
spectra of all species present in complex mixtures. In this work,
we present a novel multiplexed approach and demonstrate its utility
for cryogenic ion spectroscopy of peptides and glycans in mixtures.
Since the method is based on Hadamard transform multiplexing, it yields
infrared spectra with an increased signal-to-noise ratio compared
to a conventional signal averaging approach.