ac0156619_si_001.pdf (23.42 kB)
High-Density Fiber-Optic Genosensor Microsphere Array Capable of Zeptomole Detection Limits
journal contribution
posted on 2002-03-08, 00:00 authored by Jason R. Epstein, Myoyong Lee, David R. WaltThe detection limit of a fiber-optic microsensor array was
investigated for simultaneous detection of multiple DNA
sequences. A random array composed of oligonucleotide-functionalized 3.1-μm-diameter microspheres on the distal face of a 500-μm etched imaging fiber was monitored
for binding to fluorescently labeled complementary DNA
sequences. Inherent sensor redundancy in the microarray
allows the use of multiple microspheres to increase the
signal-to-noise ratio, further enhancing the detection
capabilities. Specific hybridization was observed for each
of three sequences in an array yielding a detection limit
of 10-21 mol (∼600 DNA molecules).