High-Density Fiber-Optic Genosensor Microsphere Array Capable of Zeptomole Detection Limits
journal contributionposted on 08.03.2002 by Jason R. Epstein, Myoyong Lee, David R. Walt
Any type of content formally published in an academic journal, usually following a peer-review process.
The detection limit of a fiber-optic microsensor array was investigated for simultaneous detection of multiple DNA sequences. A random array composed of oligonucleotide-functionalized 3.1-μm-diameter microspheres on the distal face of a 500-μm etched imaging fiber was monitored for binding to fluorescently labeled complementary DNA sequences. Inherent sensor redundancy in the microarray allows the use of multiple microspheres to increase the signal-to-noise ratio, further enhancing the detection capabilities. Specific hybridization was observed for each of three sequences in an array yielding a detection limit of 10-21 mol (∼600 DNA molecules).