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Heterotrimeric Coiled Coils with Core Residue Urea Side Chains

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journal contribution
posted on 19.12.2008, 00:00 by Maria L. Diss, Alan J. Kennan
We report several coiled coil heterotrimers with varying core residue buried polar groups, all with Tm values >43 °C. Introduction of new synthetic side chain structures, including some terminating in monosubstituted ureas, diversifies the pool of viable core residue candidates. A study of core charge pairings demonstrates that, unlike dimeric systems, trimeric coiled coils do not tolerate guanidine−guanidine contacts, even in the presence of a compensating carboxylate. Overall, the roster of feasible coiled coil designs is significantly expanded.