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Heterochiral DNA Strand-Displacement Based on Chimeric d/l-Oligonucleotides
journal contribution
posted on 2019-11-08, 20:39 authored by Brian
E. Young, Jonathan T. SczepanskiHeterochiral DNA strand-displacement
reactions enable sequence-specific
interfacing of oligonucleotide enantiomers, making it possible to
interface native d-nucleic acids with molecular circuits
built using nuclease-resistant l-DNA. To date, all heterochiral
reactions have relied on peptide nucleic acid (PNA), which places
potential limits on the scope and utility of this approach. Herein,
we now report heterochiral strand-displacement in the absence of PNA,
instead utilizing chimeric d/l-DNA complexes to interface
oligonucleotides of the opposite chirality. We show that these strand-displacement
reactions can be easily integrated into multicomponent heterochiral
circuits, are compatible with both DNA and RNA inputs, and can be
engineered to function in serum-supplemented medium. We anticipate
that these new reactions will lead to a wider application of heterochiral
strand-displacement, especially in the design of biocompatible nucleic
acid circuits that can reliably operate within living systems.
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d-nucleic acidsmulticomponent heterochiral circuitsreport heterochiral strand-displacementRNA inputsheterochiral strand-displacementacid circuitsoligonucleotide enantiomersnuclease-resistant l-DNAstrand-displacement reactionsinterface oligonucleotidesheterochiral reactionssequence-specific interfacingHeterochiral DNA Strand-DisplacementPNAserum-supplemented medium
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