posted on 2022-12-24, 00:29authored byJiao Zhang, Huijie Liu, Qihang Yu, Zhenzhen Zhan, Tong Li, Lingxin Shu, Chuxi Zhang, Haoxin Cheng, Teng Zhang, Hongbo Xin, Xiaolei Wang
The large amount of reactive oxygen species (ROS) produced
by high
glucose metabolism in diabetic patients not only induces inflammation
but also damages blood vessels, finally resulting in low limb temperature,
and the high glucose environment in diabetic patients also makes them
susceptible to bacterial infection. Therefore, diabetic foot ulcer
(DFU) usually presents as a nonhealing wound. To efficaciously prevent
and treat DFU, we proposed a near-infrared (NIR) responsive microneedle
(MN) patch hierarchical microparticle (HMP)-ZnO-MN-vascular endothelial
growth factor and basic fibroblast growth factor (H-Z-MN-VEGF&bFGF),
which could deliver drugs to the limbs painlessly, accurately, and
controllably under NIR irradiation. Therein, the hair-derived HMPs
exhibited the capacity of scavenging ROS, thereby preventing damage
to the blood vessels. Meanwhile, zinc oxide (ZnO) nanoparticles endowed
the MN patch with excellent antibacterial activity which could be
further enhanced with the photothermal effect of HMPs under NIR irradiation.
Moreover, vascular endothelial growth factor and basic fibroblast
growth factor could promote the angiogenesis. A series of experiments
proved that the MN patch exhibited broad-spectrum antibacterial and
anti-inflammatory capacities. In vivo, it obviously
increased the temperature of fingertips in diabetic rats as well as
promoted collagen deposition and angiogenesis during wound healing.
In conclusion, this therapeutic platform provides a promising method
for the prevention and treatment of DFU.