posted on 2021-09-28, 13:04authored byXuelian Wang, Mei Liu, Xueqin Ding
Six hydrophobic magnetic guanidinium
ionic liquids (HMILs) were
designed and prepared for the extraction of DNA. The physical and
thermal properties of the HMILs were characterized using vibrating
sample magnetometry, density meter, rotational rheometer, Karl Fischer
moisture, Fourier transform infrared spectrometry, and thermogravimetric
analysis. Single-stranded DNA and duplex DNA extracted by HMILs can
be rapidly collected by a magnet. Three assisted extraction methods,
including vortex extraction, mechanical shaking extraction, and ultrasonic
extraction, were introduced to extract DNA with HMILs and the extraction
efficiencies were evaluated using NanoDrop. Influencing factors of
the DNA extraction were comprehensively evaluated, involving the HMIL
volume, extraction time, pH, and extraction temperature. The HMIL-based
extraction method can well extract DNA from complex matrices and Escherichia coli cell lysates.