ao0c01640_si_001.pdf (317.4 kB)
Growth-Inhibiting, Bactericidal, Antibiofilm, and Urease Inhibitory Activities of Hibiscus rosa sinensis L. Flower Constituents toward Antibiotic Sensitive- and Resistant-Strains of Helicobacter pylori
journal contribution
posted on 2020-08-07, 15:14 authored by Hieu Tran Trung, Hoa Truong Thi Huynh, Linh Nguyen Thi Thuy, Hoang Nguyen Van Minh, My-Nuong Thi Nguyen, My Ngan Luong ThiThe aim of the present
study was to assess antimicrobial effects
of naringenin (NRG), luteolin (LUT), myricetin (MCT), and protocatechuic
acid (PCA) identified in a Hibiscus rosa sinensis flower against two reference strains and five clinical isolates
of Helicobacter pylori. NRG displayed
the most growth inhibitory and bactericidal activities to seven bacterial
strains including six strains resistant to one or several antibiotics,
azithromycin (MIC, 16–32 mg/L), erythromycin (MIC, 32 mg/L),
levofloxacin (MIC, 32 mg/L), and/or metronidazole (24–64 mg/L),
followed by LUT and MCT, while PCA showed weak activities toward the
strains. These constituents had similar antibacterial activities toward
the seven tested strains suggesting that these constituents and the
antibiotics do not have a common mechanism of anti-H. pylori activity. NRG, LUT, and MCT resulted in
a high percentage of coccoid forms of H. pylori. NRG exhibited the highest anti-biofilm formation activity. MCT
produced the strongest inhibition of urease activity followed by LUT
and PCA, whereas the activity of NRG was similar to standard inhibitor
thiourea. The four constituents had no significant toxicity to human
cell lines. A global attempt to decrease utilization of antibiotics
justifies the need for further research on H. rosa
sinensis derived materials containing NRG, LUT, MCT,
and PCA as potential products or lead compounds for the prevention
or treatment of diseases caused by H. pylori infection.