Green Synthesis of Cerium Oxide Nanoparticles, Characterization,
and Their Neuroprotective Effect on Hydrogen Peroxide-Induced Oxidative
Injury in Human Neuroblastoma (SH-SY5Y) Cell Line
posted on 2024-01-03, 21:51authored byMadhugiri
Gopinath Mamatha, Mohammad Azam Ansari, M Yasmin Begum, Daruka Prasad B., Adel Al Fatease, Umme Hani, Mohammad N. Alomary, Sumreen Sultana, Shital Manohar Punekar, Nivedika M.B., Thimappa Ramachandrappa Lakshmeesha, Tekupalli Ravikiran
Cerium oxide nanoparticles
(CeO2NPs) have a broad scale
of applications in the biomedical field due to their excellent physicochemical
and catalytic properties. The present study aims to synthesize the
CeO2NPs from Centella asiatica (C. asiatica) leaf extract, which
has been used in Indian traditional medicine for its neuroprotective
properties. The CeO2NPs were characterized by ultraviolet–visible,
X-ray diffraction, Fourier transform infrared, Raman spectroscopy,
scanning electron microscopy– energy dispersive X-ray spectroscopy,
and high-resolution transmission electron microscopy. The antioxidant
property was evaluated by 2,2-di (4-tert-octyl phenyl)-1-picrylhydrazyl
and OH radical assays. The neuroprotective potential was assessed
against the oxidative stress (OS) induced by H2O2 in the human neuroblastoma (SH-SY5Y) cell line. CeO2NPs
exhibited significant DPPH and OH radical scavenging activity. Our
results revealed that CeO2NPs significantly increased H2O2-induced cell viability, decreased lactate dehydrogenase,
protein carbonyls, reactive oxygen species generation, apoptosis,
and upregulated antioxidant enzyme activity. Our findings suggest
that the CeO2NPs protect the SH-SY5Y cells from OS and
apoptosis, which could potentially counter OS-related neurodegenerative
disorders.