American Chemical Society
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Glycan-Imprinted Nanoparticle as Artificial Neutralizing Antibody for Efficient HIV‑1 Recognition and Inhibition

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journal contribution
posted on 2024-04-03, 13:35 authored by Juntao Zhou, Libian Wang, Xiaoyan Liu, Yanxin Gai, Mingming Dong, Chu Wang, Muhammad Mujahid Ali, Mingliang Ye, Xianghui Yu, Lianghai Hu
The HIV-1 envelope is a heavily glycosylated class 1 trimeric fusion protein responsible for viral entry into CD4+ immune cells. Developing neutralizing antibodies against the specific envelope glycans is an alternative method for antiviral therapies. This work presents the first-ever development and characterization of artificial neutralizing antibodies using molecular imprinting technology to recognize and bind to the envelope protein of HIV-1. The prepared envelope glycan-imprinted nanoparticles (GINPs) can successfully prevent HIV-1 from infecting target cells by shielding the glycans on the envelope protein. In vitro experiments showed that GINPs have strong affinity toward HIV-1 (Kd = 36.7 ± 2.2 nM) and possess high anti-interference and specificity. GINPs demonstrate broad inhibition activity against both tier 1 and tier 2 HIV-1 strains with a pM-level IC50 and exhibit a significant inhibitory effect on long-term viral replication by more than 95%. The strategy provides a promising method for the inhibition and therapy of HIV-1 infection.