American Chemical Society
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Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET

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journal contribution
posted on 2011-02-09, 00:00 authored by Tara Sabir, Gunnar F. Schröder, Anita Toulmin, Peter McGlynn, Steven W. Magennis
Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA−protein interaction studies.