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Download fileGenetic Characterization of Neosartorin Biosynthesis Provides Insight into Heterodimeric Natural Product Generation
journal contribution
posted on 2018-11-05, 14:42 authored by Yudai Matsuda, Charlotte H. Gotfredsen, Thomas O. LarsenA biosynthetic gene
cluster of the fungal xanthone heterodimer
neosartorin (1) was discovered in Aspergillus
novofumigatus, and its biosynthesis was investigated by a
series of gene-deletion experiments. The results indicate that the
two monomeric units of 1 are synthesized by the same
set of enzymes, with chrysophanol (5) as a common precursor.
Furthermore, the P450 monooxygenase NsrP for the heterodimerization
was discovered, which also accepts non-native substrates to afford
novel xanthone dimers.
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Keywords
chrysophanolseriesnon-native substratesbiosynthesienzymeP 450 monooxygenase NsrPAspergillus novofumigatusNeosartorin Biosynthesis Provides InsightGenetic Characterizationmonomeric unitsnovel xanthone dimersxanthone heterodimer neosartoringene-deletion experimentsheterodimerizationHeterodimeric Natural Product Generationprecursorbiosynthetic gene cluster