Genetic Characterization of Neosartorin Biosynthesis Provides Insight into Heterodimeric Natural Product Generation
journal contributionposted on 2018-11-05, 14:42 authored by Yudai Matsuda, Charlotte H. Gotfredsen, Thomas O. Larsen
A biosynthetic gene cluster of the fungal xanthone heterodimer neosartorin (1) was discovered in Aspergillus novofumigatus, and its biosynthesis was investigated by a series of gene-deletion experiments. The results indicate that the two monomeric units of 1 are synthesized by the same set of enzymes, with chrysophanol (5) as a common precursor. Furthermore, the P450 monooxygenase NsrP for the heterodimerization was discovered, which also accepts non-native substrates to afford novel xanthone dimers.
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chrysophanolseriesnon-native substratesbiosynthesienzymeP 450 monooxygenase NsrPAspergillus novofumigatusNeosartorin Biosynthesis Provides InsightGenetic Characterizationmonomeric unitsnovel xanthone dimersxanthone heterodimer neosartoringene-deletion experimentsheterodimerizationHeterodimeric Natural Product Generationprecursorbiosynthetic gene cluster