posted on 2020-03-19, 21:29authored byHuijuan Guo, Jan W. Schwitalla, René Benndorf, Martin Baunach, Christoph Steinbeck, Helmar Görls, Z. Wilhelm de Beer, Lars Regestein, Christine Beemelmanns
Growth from spores
activated a biosynthetic gene cluster in Actinomadura sp. RB29, resulting in the identification of
two novel groups of halogenated polyketide natural products, named
maduralactomycins and actinospirols. The unique tetracyclic and spirocyclic
structures were assigned based on a combination of NMR analysis, chemoinformatic
calculations, X-ray crystallography, and 13C labeling studies.
On the basis of HRMS2 data, genome mining, and gene expression
studies, we propose an underlying noncanonical angucycline biosynthesis
and extensive post-polyketide synthase (PKS) oxidative modifications.