ao6b00044_si_001.pdf (473.66 kB)
Folding Kinetics of Single Human Telomeric G‑Quadruplex Affected by Cisplatin
journal contribution
posted on 2016-08-03, 00:00 authored by Hai-Peng Ju, Yi-Zhou Wang, Jing You, Xi-Miao Hou, Xu-Guang Xi, Shuo-Xing Dou, Wei Li, Peng-Ye WangG-Quadruplex
DNA structure has been proven to be a binding target
for small molecular organic compounds and hence regarded as a promising
pharmacological target. Cisplatin is a widely used chemotherapy drug
that targets duplex DNA and was recently shown to react also with
G-quadruplex, implying that cisplatin actually may also target G-quadruplex.
In this work, we employed magnetic tweezers to investigate the influence
of cisplatin on the folding kinetics of single human telomeric G-quadruplex.
It was revealed that cisplatin and G-quadruplex interact in two different
and competitive ways that depend on cisplatin concentration.