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Fluorescent Probe for in Vivo Partitioning into Dynamic Lipid Droplets Enables Monitoring of Water Permeability-Induced Edema
journal contributionposted on 2023-07-21, 12:06 authored by Cinthia Hernández-Juárez, Gilberto Morales-Villafaña, Fernando López-Casillas, Arturo Jiménez-Sánchez
Lipid droplets (LDs) are intracellular organelles found in most cell types from adipocytes to cancer cells. Although recent investigations have implicated LDs in numerous diseases, the current available methods to monitor them in vertebrate models rely on static imaging using fluorescent dyes, limiting the investigation of their rapid in vivo dynamics. Here, we report a fluorophore chemistry approach to enable in vivo LD dynamic monitoring using a Nernstian partitioning mechanism. Interestingly, the effect of atorvastatin and osmotic treatments toward LDs revealed an unprecedented dynamic enhancement. Then, using a designed molecular probe with an optimized response to hydration and LD dynamics applied to Zebrafish developing pericardial and yolk-sac edema, which represents a tractable model of a human cardiovascular disease, we also provide a unique dual method to detect disease evolution and recovery.
zebrafish developing pericardialvertebrate models relyunprecedented dynamic enhancementunique dual methodintracellular organelles foundhuman cardiovascular diseasefluorophore chemistry approachdetect disease evolutioncurrent available methodsalthough recent investigationsnernstian partitioning mechanismld dynamics applieddesigned molecular probevivo partitioningvivo dynamicsfluorescent probewater permeabilitytractable modelsac edemaoptimized responsenumerous diseasescell typescancer cellsalso provide