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Fluorescent 1,10-Phenanthroline-Containing Oligonucleotides Distinguish between Perfect and Mismatched Base Pairing

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journal contribution
posted on 08.06.2002, 00:00 by Dennis J. Hurley, Susan E. Seaman, Jan C. Mazura, Yitzhak Tor
A fluorescent deoxyuridine analogue is sensitive to the polarity of its environment and exhibits a distinct emission profile in single- vs double-stranded oligonucleotides. Emission-monitored denaturation curves of internally modified dUphen duplexes are characteristic of the base opposite dUphen and distinguish between perfect and mismatched complementary oligonucleotides.