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Fluorescence Probes Exhibit Photoinduced Structural Planarization: Sensing In Vitro and In Vivo Microscopic Dynamics of Viscosity Free from Polarity Interference
journal contribution
posted on 2020-06-30, 15:08 authored by Cheng-Ham Wu, Yi Chen, Kyrylo A. Pyrshev, Yi-Ting Chen, Zhiyun Zhang, Kai-Hsin Chang, Semen O. Yesylevskyy, Alexander P. Demchenko, Pi-Tai ChouWe
demonstrate the construction of wavelength λ-ratiometric
images that allow visualizing the distribution of microscopic dynamics
within living cells and tissues by using the newly developed principle
of fluorescence response. The bent-to-planar motion in the excited
state of incorporated fluorescence probes leads to elongation of the
π-delocalization, resulting in microviscosity-dependent but
polarity-insensitive interplay between well-separated blue and red
bands in emission spectra. This allows constructing the exceptionally
contrasted images of cellular dynamics. Moreover, the application
of probes with increased affinity toward biological membranes allowed
detecting the differences in dynamics between the plasma membrane
and intracellular membrane structures. Such λ-ratiometric microviscosity
imaging was extended for mapping the living tissues and observing
their inflammation-dependent changes.
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fluorescence responseemission spectratissueplasma membranefluorescence probesSuch λ- ratiometric microviscosity ...inflammation-dependent changesbent-to-planar motionPolarity Interferencewavelength λ- ratiometric imagesintracellular membrane structuresdynamicπ- delocalizationFluorescence Probes Exhibit Photoin...Viscosity Freepolarity-insensitive interplay
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