posted on 2016-02-21, 17:04authored byAnton
N. Kozhinov, Yury O. Tsybin
Molecular and macromolecular structure analysis by high
resolution
and accurate mass spectrometry (MS) is indispensable for a number
of fundamental and applied research areas, including health and energy
domains. Comprehensive structure analysis of molecules and macromolecules
present in the extremely complex samples and performed under time-constrained
experimental conditions demands a substantial increase in the acquisition
speed of high resolution MS data. We demonstrate here that signal
processing based on the filter diagonalization method (FDM) provides
the required resolution for shorter experimental transient signals
in ion cyclotron resonance (ICR) MS compared to the Fourier transform
(FT) processing. We thus present the development of a FDM-based MS
(FDM MS) and demonstrate its implementation in ICR MS. The considered
FDM MS applications are in bottom-up and top-down proteomics, metabolomics,
and petroleomics.