nl1c02702_si_001.pdf (506.81 kB)
Ferumoxytol Nanoparticles Target Biofilms Causing Tooth Decay in the Human Mouth
journal contributionposted on 2021-10-15, 23:43 authored by Yuan Liu, Yue Huang, Dongyeop Kim, Zhi Ren, Min Jun Oh, David P. Cormode, Anderson T. Hara, Domenick T. Zero, Hyun Koo
Severe tooth decay has been associated with iron deficiency anemia that disproportionally burdens susceptible populations. Current modalities are insufficient in severe cases where pathogenic dental biofilms rapidly accumulate, requiring new antibiofilm approaches. Here, we show that ferumoxytol, a Food and Drug Administration-approved nanoparticle formulation for treating iron deficiency, exerts an alternative therapeutic activity via the catalytic activation of hydrogen peroxide, which targets bacterial pathogens in biofilms and suppresses tooth enamel decay in an intraoral human disease model. Data reveal the potent antimicrobial specificity of ferumoxytol iron oxide nanoparticles (FerIONP) against biofilms harboring Streptococcus mutans via preferential binding that promotes bacterial killing through in situ free-radical generation. Further analysis indicates that the targeting mechanism involves interactions of FerIONP with pathogen-specific glucan-binding proteins, which have a minimal effect on commensal streptococci. In addition, we demonstrate that FerIONP can detect pathogenic biofilms on natural teeth via a facile colorimetric reaction. Our findings provide clinical evidence and the theranostic potential of catalytic nanoparticles as a targeted anti-infective nanomedicine.
treating iron deficiencytargets bacterial pathogenspromotes bacterial killingpotent antimicrobial specificitynatural teeth viairon deficiency anemiafacile colorimetric reactionapproved nanoparticle formulationvia preferential bindingstreptococcus mutans detect pathogenic biofilmssitu binding proteinstheranostic potentialtargeted antispecific glucansevere casesradical generationminimal effectinfective nanomedicinehydrogen peroxidedrug administrationdata revealcurrent modalitiescommensal streptococcicatalytic nanoparticlescatalytic activationbiofilms harboringanalysis indicates