jf5b05998_si_001.pdf (105.43 kB)
Fate of Fusarium Toxins during the Malting Process
journal contribution
posted on 2016-01-27, 00:00 authored by Katharina Habler, Katharina Hofer, Cajetan Geißinger, Jan Schüler, Ralph Hückelhoven, Michael Hess, Martina Gastl, Michael RychlikLittle is known about the fate of Fusarium mycotoxins during the barley malting process.
To determine the fungal
DNA and mycotoxin concentrations during malting, we used barley grain
harvested from field plots that we had inoculated with Fusarium species that produce type A or type B trichothecenes
or enniatins. Using a recently developed multimycotoxin liquid chromatography–tandem
mass stable isotope dilution method, we identified Fusarium-species-specific behaviors of mycotoxins
in grain and malt extracts and compared toxin concentrations to amounts
of fungal DNA in the same samples. In particular, the type B trichothecenes
and Fusarium culmorum DNA contents
were increased dramatically up to 5400% after kilning. By contrast,
the concentrations of type A trichothecenes and Fusarium
sporotrichioides DNA decreased during the malting
process. These data suggest that specific Fusarium species that contaminate the raw grain material might have different
impacts on malt quality.