Fabrication of
an Antifouling Surface Plasmon Resonance
Sensor with Stratified Zwitterionic Peptides for Highly Efficient
Detection of Peanut Allergens in Biscuits
posted on 2024-05-01, 16:34authored byYinqiang Xia, Xinru Dong, Heng Chang, Xiwen Zhang, Jinyu Li, Siqi Wang, Yang Lu, Tianli Yue
Peanut allergen monitoring is currently an effective
strategy to
avoid allergic diseases, while food matrix interference is a critical
challenge during detection. Here, we developed an antifouling surface
plasmon resonance sensor (SPR) with stratified zwitterionic peptides,
which provides both excellent antifouling and sensing properties.
The antifouling performance was measured by the SPR, which showed
that stratified peptide coatings showed much better protein resistance,
reaching ultralow adsorption levels (<5 ng/cm2). Atomic
force microscopy was used to further analyze the antifouling mechanism
from a mechanical perspective, which demonstrated lower adsorption
forces on hybrid peptide coatings, confirming the better antifouling
performance of stratified surfaces. Moreover, the recognition of peanut
allergens in biscuits was performed using an SPR with high efficiency
and appropriate recovery results (98.2–112%), which verified
the feasibility of this assay. Therefore, the fabrication of antifouling
sensors with stratified zwitterionic peptides provides an efficient
strategy for food safety inspection.