bm7b00406_si_001.pdf (972.91 kB)
Download fileFabrication of Silk Scaffolds with Nanomicroscaled Structures and Tunable Stiffness
journal contribution
posted on 2017-06-02, 00:00 authored by Liying Xiao, Shanshan Liu, Danyu Yao, Zhaozhao Ding, Zhihai Fan, Qiang Lu, David L. KaplanDetailed
control of nano- and microstructures in porous biomaterial
scaffold systems is important for control of interfacial and biological
functions. Self-assembled silk protein nanostructured building blocks
were incorporated into salt-leached scaffolds to control these features.
Controllable concentration and pH were used to induce the formation
of amorphous silk nanofibers in solution and also to reduce β-sheet
transformation during the more traditional salt-leaching process.
These new scaffolds showed nanofibrous-microporous structures, reduced
β-sheet content, and tunable mechanical properties. Bone marrow
mesenchymal stem cells grew better and showed differentiation behavior
on these nanofibrous scaffolds, suggesting cytocompatibility and support
for tunable differentiation via the scaffolds. These results suggested
a new strategy of designing bioactive silk scaffolds by combining
traditional scaffold formation processes with the controllable self-assembly
of silk.
History
Usage metrics
Read the peer-reviewed publication
Categories
Keywords
β- sheet contentnanofibrous-microporous structuressalt-leaching processSilk ScaffoldsSelf-assembled silk protein nanostructured building blocksbiomaterial scaffold systemsNanomicroscaled StructuresTunable Stiffnessscaffold formation processesdifferentiation behaviornanofibrous scaffoldsbioactive silk scaffoldstunable differentiationBone marrow mesenchymalβ- sheet transformationControllable concentrationsalt-leached scaffoldssilk nanofibers