FRET Luminescent
Probe for the Ratiometric Imaging
of Peroxynitrite in Rat Brain Models of Epilepsy-Based on Organic
Dye-Conjugated Iridium(III) Complex
posted on 2023-12-04, 17:03authored byXiaona Gao, Wenzhu Zhang, Zhiyuan Dong, Junyu Ren, Bo Song, Run Zhang, Jingli Yuan
Epilepsy is a chronic neurological disorder characterized
by recurrent
seizures globally, imposing a substantial burden on patients and their
families. The pathological role of peroxynitrite (ONOO–), which can trigger oxidative stress, inflammation, and neuronal
hyperexcitability, is critical in epilepsy. However, the development
of reliable, in situ, and real-time optical imaging tools to detect
ONOO– in the brain encounters some challenges related
to the depth of tissue penetration, background interference, optical
bleaching, and spectral overlapping. To address these limitations,
we present Ir-CBM, a new one-photon and two-photon excitable
and long-lived ratiometric luminescent probe designed specifically
for precise detection of ONOO– in epilepsy-based
on the Förster resonance energy transfer mechanism by combining
an iridium(III) complex with an organic fluorophore. Ir-CBM possesses the advantages of rapid response, one-/two-photon excitation,
and ratiometric luminescent imaging for monitoring the cellular levels
of ONOO– and evaluating the effects of different
therapeutic drugs on ONOO– in the brain of an epilepsy
model rat. The development and utilization of Ir-CBM offer
valuable insights into the design of ratiometric luminescent probes.
Furthermore, Ir-CBM serves as a rapid imaging and screening
tool for antiepileptic drugs, thereby accelerating the exploration
of novel antiepileptic drug screening and improving preventive and
therapeutic strategies in epilepsy research.