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Download fileEvaluation of Nitric Oxide Fluctuation Via a Fast, Responsive Fluorescent Probe in Idiopathic Pulmonary Fibrosis Cells and Mice Models
journal contribution
posted on 2022-02-23, 13:35 authored by Feifei Xu, Qing Wang, Ling Jiang, Fawei Zhu, Lei Yang, Shusheng Zhang, Xiangzhi SongIdiopathic
pulmonary fibrosis (IPF) is a chronic, progressive,
and fatal interstitial pneumonia with unknown pathogenesis. Early
diagnosis and therapeutic intervention are essential for improving
the prognosis of patients with IPF. The level of nitric oxide upregulates
in the alveoli of IPF patients, which is correlated with the severity
of the disease. Herein, we report a fluorescent probe DCM–nitric
oxide (NO) to detect IPF by monitoring the concentration changes of
NO. This probe displays a fast response time and a good linear response
to NO in vitro. Fluorescence imaging experiments with probe DCM–NO
revealed that the level of intracellular NO increases in the pulmonary
fibrosis cells and IPF mice models. Probe DCM–NO displayed
a strong red fluorescence in IPF mice models. However, a declining
fluorescence was evidenced in the OFEV-treated IPF mice, implying
that DCM–NO is capable of evaluating the therapeutic effects
on IPF. Thus, probe DCM–NO can quickly predict the progression
of pulmonary fibrosis at an early stage and thus help improve the
effective treatment.
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nitric oxide upregulatesgood linear responsefatal interstitial pneumoniastrong red fluorescenceresponsive fluorescent probefluorescence imaging experimentstreated ipf miceipf mice modelsthus help improvepulmonary fibrosis cellsfast response timeprobe dcm –pulmonary fibrosisdcm –probe displaysdeclining fluorescencedetect ipfunknown pathogenesistherapeutic interventiontherapeutic effectsquickly predicteffective treatmentearly stageearly diagnosisconcentration changes