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Ethane-Bridged Bisporphyrin Conformational Changes As an Effective Analytical Tool for Nonenzymatic Detection of Urea in the Physiological Range
journal contribution
posted on 2018-05-04, 00:00 authored by Alessandro Buccolieri, Mohammed Hasan, Simona Bettini, Valentina Bonfrate, Luca Salvatore, Angelo Santino, Victor Borovkov, Gabriele GiancaneConformational
switching induced in ethane-bridged bisporphyrins
was used as a sensitive transduction method for revealing the presence
of urea dissolved in water via nonenzymatic approach. Bisporphyrins
were deposited on solid quartz slides by means of the spin-coating
method. Molecular conformations of Zn and Ni monometalated bis-porphyrins
were influenced by water solvated urea molecules and their fluorescence
emission was modulated by the urea concentration. Absorption, fluorescence
and Raman spectroscopies allowed the identification of supramolecular
processes, which are responsible for host–guest interaction
between the active layers and urea molecules. A high selectivity of
the sensing mechanism was highlighted upon testing the spectroscopic
responses of bis-porphyrin films to citrulline and glutamine used
as interfering agents. Additionally, potential applicability was demonstrated
by quantifying the urea concentration in real physiological samples
proposing this new approach as a valuable alternative analytical procedure
to the traditionally used enzymatic methods.