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Enzymatic Reduction of Ketones in “Micro-aqueous” Media Catalyzed by ADH-A from Rhodococcus ruber

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journal contribution
posted on 24.05.2007, 00:00 by Gonzalo de Gonzalo, Iván Lavandera, Kurt Faber, Wolfgang Kroutil
Mono- and biphasic aqueous−organic solvent systems (50% v v-1) as well as micro-aqueous organic systems (99% v v-1) were successfully employed for the biocatalytic reduction of ketones catalyzed by alcohol dehydrogenase ADH-A from Rhodococcus ruber via hydrogen transfer. A clear correlation between the log P of the organic solvent and the enzyme activitythe higher, the betterwas found. The use of organic solvents allowed highly stereoselective enzymatic carbonyl reductions at substrate concentrations close to 2.0 M.