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Enhanced Accumulation of Phytosterol and Triterpene in Hairy Root Cultures of Platycodon grandiflorum by Overexpression of Panax ginseng 3‑Hydroxy-3-methylglutaryl-coenzyme A Reductase
journal contributionposted on 2016-02-19, 21:24 authored by Yong-Kyoung Kim, Jae Kwang Kim, Yeon Bok Kim, Sanghyun Lee, Soo-Un Kim, Sang Un Park
3-Hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyzes the rate-limiting step in the mevalonate pathway. To elucidate the functions of HMGR in triterpene biosynthesis, Platycodon grandiflorum was transformed with a construct expressing Panax ginseng HMGR (PgHMGR). We used PCR analysis to select transformed hairy root lines and selected six lines for further investigation. Quantitative real-time PCR showed higher expression levels of HMGR and total platycoside levels (1.5–2.5-fold increase) in transgenic lines than in controls. Phytosterols levels were also 1.1–1.6-fold higher in transgenic lines than in controls. Among these lines, line T7 produced the highest level of total platycosides (1.60 ± 0.2 mg g–1 dry weight) and α-spinasterol (1.78 ± 0.16 mg g–1 dry weight). These results suggest that metabolic engineering of P. grandiflorum by Agrobacterium-mediated genetic transformation may enhance production of phytosterols and triterpenoids.