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Download fileEngineered Phage Matrix Stiffness-Modulating Osteogenic Differentiation
journal contribution
posted on 18.01.2018, 00:00 authored by Hee-Sook Lee, Jeong-In Kang, Woo-Jae Chung, Do Hoon Lee, Byung Yang Lee, Seung-Wuk Lee, So Young YooHerein, we demonstrate
an engineered phage mediated matrix for osteogenic differentiation
with controlled stiffness by cross-linking the engineered phage displaying
Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations
of streptavidin or polymer, poly(diallyldimethylammonium)chloride
(PDDA). Osteogenic gene expressions showed that they were specifically
increased when MC3T3 cells were cultured on the stiffer phage matrix
than the softer one. Our phage matrixes can be easily functionalized
using chemical/genetic engineering and used as a stem cell tissue
matrix stiffness platform for modulating differential cell expansion
and differentiation.