Version 2 2024-01-23, 18:34Version 2 2024-01-23, 18:34
Version 1 2024-01-23, 00:29Version 1 2024-01-23, 00:29
journal contribution
posted on 2024-01-23, 18:34authored byWeiming Chen, Jiale Sun, Yuqing Mao, Yuhao Tang, Jing Wang, Zhihong Liu
Tumor-derived extracellular vesicle (T-EV) microRNAs
have been
investigated as promising biomarkers in clinical diagnosis as well
as disease progression monitoring. However, the expression profiles
of microRNA in different tissues vary widely, the precise monitoring
of microRNA levels in EVs originating from diseased tissues is susceptible
to background interference, thus remains a challenge. Conventional
assays require extensive processing, such as EV isolation and even
sample lysis, which is both slow and laborious, and the cumbersome
pretreatment could spoil the downstream analysis. To address this
issue, we developed a generalizable strategy for T-EVs-selective activation
and therefore specific amplified microRNA imaging. The conditional
signal amplification is established by integrating a traditional DNA
walker system with endogenously activated motif to achieve sensitized
microRNA imaging in T-EVs. The preorganized endogenous activation
with additional sensing criteria narrowed the scope against the complex
specimens, and the amplified sensing with reduced off-target signals
was supposed to be sensitive to monitor the tiny changes of microRNA
expression during the disease course, which holds great potential
for accurate diagnosis and prognosis.