mp300458h_si_001.pdf (280.16 kB)
Endocytic Pathway and Resistance to Cholesterol Depletion of Cholesterol Derived Cationic Lipids for Gene Delivery
journal contribution
posted on 2012-12-03, 00:00 authored by Yun-Ui Bae, Bieong-Kil Kim, Jong-Won Park, Young-Bae Seu, Kyung-Oh DohCholesterol-based cationic lipids have been widely used
because
of biocompatibility and serum resistance. However, the reason for
the effectiveness of cholesterol-based cationic lipids remains unclear.
We compared the transfection route of CHOL-E, a cholesterol-based
cationic lipid having an amine head and an ether linker, with that
of DOTAP. The luciferase assay with chemical inhibitors and microscopic
observation of pathway markers revealed that clathrin mediated endocytosis
is the main pathway for CHOL-E and DOTAP. However, CHOL-E showed resistance
to cholesterol depletion by methyl-β-cyclodextrin. Furthermore,
CHOL-E recovered the transfection efficiency of DOTAP from cholesterol
depletion. These results suggested that superior transfection of CHOL-E
might be partly derived from effects on the cell membrane.