American Chemical Society
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Electrospun Nanofibrous UV Filters with Bidirectional Actuation Properties Based on Salmon Sperm DNA/Silk Fibroin for Biomedical Applications

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journal contribution
posted on 2023-10-04, 23:33 authored by Maria Rachele Ceccarini, Irene Chiesa, Francesca Ripanti, Martina Alunni Cardinali, Simone Micalizzi, Gabriele Scattini, Carmelo De Maria, Alessandro Paciaroni, Caterina Petrillo, Lucia Comez, Matteo Bertelli, Paola Sassi, Luisa Pascucci, Tommaso Beccari, Luca Valentini
In this study, we dissolved Bombyx mori degummed silk [i.e., silk fibroin (SF)] and salmon sperm deoxyribonucleic acid (DNA) in water and used a bioinspired spinning process to obtain an electrospun nanofibrous SF-based patch (ESF). We investigated the bidirectional macroscale actuation behavior of ESF in response to water vapor and its UV-blocking properties as well as those of ESF/DNA films. Fourier transform infrared (FTIR) results suggest that the formation of β-sheet-rich structures promotes the actuation effect. ESF/DNA film with high-ordered and β-sheet-rich structures exhibits higher electrical conductivity and is water-insoluble. Given the intrinsic ability of both SF and DNA to absorb UV radiation, we performed biological experiments on the viability of keratinocyte HaCaT cells after exposure to solar spectrum components. Our findings indicate that the ESF/DNA patch is photoprotective and can increase the cellular viability of keratinocytes after UV exposure. Furthermore, we demonstrated that ESF/DNA patches treated with water vapor can serve as suitable scaffolds for tissue engineering and can improve tissue regeneration when cellularized with HaCaT cells. The 3D shape morphing capability of these patches, along with their potential as UV filters, could offer significant practical advantages in tissue engineering.