Electrokinetic Biomolecule Preconcentration Using Xurography-Based Micro-Nano-Micro Fluidic Devices

In this paper we introduce a low cost rapid prototyping framework for designing Micro-Nano-Micro (MNM) fluidic preconcentration device based on ion concentration polarization (ICP) phenomenon. Xurography-based microchannels are separated by a strip of ion perm-selective Nafion membrane which plays the role of nanofluidic potential barrier for the negatively charged molecules. As a result, by using this rapid and inexpensive fabrication technique, it is possible to get preconcentration plugs as high as 5000 fold with an original symmetric electroosmotic flow (EOF) condition. Due to its simplicity and performance, this device could be implemented in various bioanalysis systems.