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Download fileElectrochemiluminescence Detection of Escherichia coli O157:H7 Based on a Novel Polydopamine Surface Imprinted Polymer Biosensor
journal contribution
posted on 2017-01-18, 00:00 authored by Shufan Chen, Xueqian Chen, Lijuan Zhang, Juanjuan Gao, Qiang MaIn this paper, a facilely prepared
electrochemiluminescence (ECL) biosensor was developed for Escherichia coli O157:H7 quantitative detection based on
a polydopamine (PDA) surface imprinted polymer (SIP) and nitrogen-doped
graphene quantum dots (N-GQDs). N-GQDs with a high quantum yield of
43.2% were synthesized. The uniform PDA SIP film for E. coli O157:H7 was established successfully with a facile route. The dopamine
and target bacteria were electropolymerized directly on the electrode.
After removal of the E. coli O157:H7 template, the
established PDA SIP can selectively recognize E. coli O157:H7. Accordingly, E. coli O157:H7 polyclonal
antibody (pAb) was labeled with N-GQDs. The bioconjugation of SIP–E. coli O157:H7/pAb-N-GQDs can generate intensive ECL irradiation
with K2S2O8. As a result, E. coli O157:H7 was detected with the ECL sensing system.
Under optimal conditions, the linear relationships between the ECL
intensity and E. coli O157:H7 concentration were
obtained from 101 colony-forming units (CFU) mL–1 to 107 CFU mL–1 with a limit of detection
of 8 CFU mL–1. The biosensor based on this SIP film
was applied in water sample detection successfully. The N-GQD-based
ECL analytical method for E. coli O157:H7 was reported
for the first time. The sensing system had high selectivity to the
target analyte, provided new opportunities for use, and increased
the rate of disease diagnosis and treatment and the prevention of
pathogens.
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Keywords
uniform PDA SIP filmwater sample detectionK 2 S 2 O 8colitarget bacteriaPDA SIPECL irradiationElectrochemiluminescence Detectiontarget analyteN-GQD-based ECLSIP filmECL intensity10 1 colony-forming unitsnitrogen-doped graphene quantum dotsNovel Polydopamine Surface Imprinted Polymer Biosensordisease diagnosisCFU