posted on 2004-06-24, 00:00authored byQing Dai, Joseph A. Piccirilli
Metal ion rescue experiments provide a powerful approach to establish the presence and role of divalent metal ions in the biological function
of RNA. The utility of this approach depends on the availability of suitable nucleoside analogues. To expand the range of this experimental
strategy, we describe the first synthesis of 2‘,3‘-dideoxy-2‘-amino-3‘-thiouridine (12) in 19.5% overall yield starting from 2,2‘-anhydrouridine
(1).