American Chemical Society
es9015519_si_001.pdf (158.76 kB)

Effects of Substrate Diffusion and Anode Potential on Kinetic Parameters for Anode-Respiring Bacteria

Download (158.76 kB)
journal contribution
posted on 2009-10-01, 00:00 authored by Hyung-Sool Lee, César I. Torres, Bruce E. Rittmann
The substrate-utilization rate of anode-respiring bacteria (ARB) directly correlates to the current density, one of the main factors in a microbial electrolysis/fuel cell. This study first evaluates the effects of donor-substrate diffusion and anode potential on the estimation of the half-maximum-rate concentration (Ks) and the maximum specific substrate-utilization rate (qmax) of a mixed culture biofilm in a microbial electrolysis cell oxidizing acetate. The intrinsic Ks value is 119 g COD/m3, substrate diffusion has a significant impact on Ks estimation, and the effect of the anode potential on Ks is small. The intrinsic qmax value is 22.3 g COD/g VS-d for an assumed biomass density of 50,000 g VS/m3 (qmaxXf = 1120 kg COD/m3-d). The maximum specific growth rate (μmax) is 3.2/d which is substantially faster than for acetate-utilizing methanogens and homoacetogens. Although the anode potential affects qmax, substrate diffusion has a negligible effect. The measured half-saturation anode potential (EKA) is very negative, −0.448 V (vs Ag/AgCl), and this low value minimizes anode-potential limitation on the current density and the substrate-utilization rate. Thus, the ARB selected in our biofilm anode were relatively fast growers able to take advantage of their low EKA value (−0.448 V).