Effects of Histidine
Supplementation on Global Serum
and Urine 1H NMR-based Metabolomics and Serum Amino Acid
Profiles in Obese Women from a Randomized Controlled Study
posted on 2017-04-27, 00:00authored byShanshan Du, Shuhong Sun, Liyan Liu, Qiao Zhang, Fuchuan Guo, Chunlong Li, Rennan Feng, Changhao Sun
The
aim of current study was to investigate the metabolic changes
associated with histidine supplementation in serum and urine metabolic
signatures and serum amino acid (AA) profiles. Serum and urine 1H NMR-based metabolomics and serum AA profiles were employed
in 32 and 37 obese women with metabolic syndrome (MetS) intervened
with placebo or histidine for 12 weeks. Multivariable statistical
analysis were conducted to define characteristic metabolites. In serum 1H NMR metabolic profiles, increases in histidine, glutamine,
aspartate, glycine, choline, and trimethylamine-N-oxide (TMAO) were observed; meanwhile, decreases in cholesterol,
triglycerides, fatty acids and unsaturated lipids, acetone, and α/β-glucose
were exhibited after histidine supplement. In urine 1H
NMR metabolic profiles, citrate, creatinine/creatine, methylguanidine,
and betaine + TMAO were higher, while hippurate was lower in histidine
supplement group. In serum AA profiles, 10 AAs changed after histidine
supplementation, including increased histidine, glycine, alanine,
lysine, asparagine, and tyrosine and decreased leucine, isoleucine,
ornithine, and citrulline. The study showed a systemic metabolic response
in serum and urine metabolomics and AA profiles to histidine supplementation,
showing significantly changed metabolism in AAs, lipid, and glucose
in obese women with MetS.