jf4004785_si_001.pdf (1.71 MB)
Effect of a Peat Humic Acid on Morphogenesis in Leaf Explants of Pyrus communis and Cydonia oblonga. Metabolomic Analysis at an Early Stage of Regeneration
journal contribution
posted on 2013-05-29, 00:00 authored by Grazia Marino, Valeria Righi, Andrea Simoni, Luisa Schenetti, Adele Mucci, Vitaliano Tugnoli, Enrico Muzzi, Ornella FranciosoPlant
regeneration is a critical step in most in vitro breeding
techniques. This paper studies the effects of a low-molecular-weight
humic acid (HA) on morphogenesis from pear and quince leaf explants.
Variable HA amounts [0 (control), 1, 5, 10, and 20 mg C L–1] were added to the regeneration media. A dose–response effect
was observed in pear for root and shoot production; it was improved
at HA 1 mg C L–1 and considerably reduced at the
highest amounts. HA was, instead, ineffective in quince. The 1H HR-MAS NMR analyses of calli in the induction phase showed
more evident metabolite (asparagine, alanine, and γ-aminobutyric
acid) signals in quince than in pear. The assignment of overlapped
signals in both genotypes was supported by the 2D NMR analyses. Spectroscopic
characterization suggested also an enhancement of asparagine contents
in morphogenic calli of pear with respect to the control and higher
HA amount treatments.