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Effect of 7Hch Hordeum chilense Chromosome Introgressions on the Wheat Endosperm Proteomic Profile

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journal contribution
posted on 15.04.2015, 00:00 by Melania Collado-Romero, Enriqueta Alós, Pilar Prieto
Hordeum chilense is an excellent genetic resource for wheat breeding due to its potential to improve breadmaking quality and nutritional value and provide resistance to some biotic and abiotic stresses. Hexaploid wheat lines carrying chromosome 7Hch introgressions, namely, chromosome additions of the whole chromosome 7Hch or the 7Hchα or the 7Hchβ chromosome arms, and chromosome substitutions of the homeologous chromosomes 7A, 7B, or 7D by chromosome 7Hch were compared by 2D-PAGE analysis to study the effect of these alien introgressions on the wheat endosperm proteome. The addition of the 7Hchα chromosome arm did not alter the profile of most glutenins and gliadins, but showed higher quantities of puroindolines and lower xylanase inhibitors, which might improve also resistance to plant pathogens. On the other hand, (7A)­7Hch or (7D)­7Hch substitution lines showed enhanced avenin-like b proteins and triticin levels but reduced puroindolines, which could be desirable to improve dough properties and nutritional value and increase kernel hardness in wheat.