American Chemical Society
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E‑Cigarette Flavoring Chemicals Induce Cytotoxicity in HepG2 Cells

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journal contribution
posted on 2021-03-02, 17:04 authored by Brittany P. Rickard, Henry Ho, Jacqueline B. Tiley, Ilona Jaspers, Kim L. R. Brouwer
E-cigarette-related hospitalizations and deaths across the U.S. continue to increase. A high percentage of patients have elevated liver function tests indicative of systemic toxicity. This study was designed to determine the effect of e-cigarette chemicals on liver cell toxicity. HepG2 cells were exposed to flavoring chemicals (isoamyl acetate, vanillin, ethyl vanillin, ethyl maltol, l-menthol, and trans-cinnamaldehyde), propylene glycol, and vegetable glycerin mixtures, and cell viability was measured. Data revealed that vanillin, ethyl vanillin, and ethyl maltol decreased HepG2 cell viability; repeated exposure caused increased cytotoxicity relative to single exposure, consistent with the hypothesis that frequent vaping can cause hepatotoxicity.