posted on 2020-09-21, 18:39authored byMarian
G. Rofeal, Ahmed O. Elzoghby, Maged W. Helmy, Rowaida Khalil, Heba Khairy, Sanaa Omar
In
view of the promising applications of nanoparticles in drug
delivery, this study highlights the fabrication of new bioactive green
protein–polysaccharide nanocomplexes with significant antibacterial
and antitumor efficacies. We preformulated the water-insoluble drugs
Quercetin (Quer) and Resveratrol (Res) as water-soluble nanocrystals
to facilitate their entrapment in the electrostatic lactoferrin–chondroitin
(Lf-ChS) nanocomplex. Quer and Res were physically entrapped in the
Lf-ChS nanomatrix with high encapsulation efficiencies (EE %) of 85.2
and 90.1% w/w for Quer and Res, respectively. The in vitro synergetic antibacterial effects of the studied compounds against
all bacterial strains were confirmed. Res-Quer Lf-ChS NPs revealed
an enhanced cytotoxic effect against A549 lung cancer cells. A new
model of polymicrobial lung infection was designed, where treatment
with Res-Quer Lf-ChS NPs (233.5 ± 6.59 nm) resulted in a marked
decline of 3.2 log units in bacterial counts. In the lung tumor model,
the potent antitumor efficacy of the developed Res-Quer Lf-ChS NPs
was demonstrated by a noticeable decline in both lung weight and the
biomarkers compared to the positive control group that did not receive
any treatment. In conclusion, the green Res-Quer Lf-ChS NPs possess
antibacterial and antitumor attributes for potential lung infection
and tumor therapy.