posted on 2024-02-21, 17:03authored byYuan Mei, Zijuan Hai, Ziyun Li, Kuanrong Rong, Wenjian Tang, Qin-Hua Song
Fluoride
anions (F–) play a crucial role in human
physiological processes. However, excessive intake of F– would affect oxygen metabolism and promote the generation of oxygen-free
radicals. Hence, it is essential to develop a precise and efficient
fluorescent probe for visualizing F–-induced oxidative
stress. In this work, we developed the first bifunctional BODIPY-based
fluorescent probe dfBDP with p-tert-butyldimethylsilanolate benzyl thioether as the sensing site for
the detection of F– and HClO via two distinct reactions,
the self-immolative removal and the thioether oxidation, which generate
the sensing products with two nonoverlap fluorescence bands: 800–1200
and 500–750 nm, respectively. The probe dfBDP displays rapid
response, high specificity, and sensitivity for the detection of F– (LOD, 316.2 nM) and HClO (LOD, 33.9 nM) in vitro.
Cellular imaging reveals a correlation between F–-induced oxidative stress and the upregulation of HClO. Finally,
probe dfBDP was employed to detect F– and HClO in
mice under the stimulation of F–. The experimental
results display that the level of HClO elevates in the liver of mice.