posted on 2021-09-13, 21:03authored byWei Lin, Gary C.H. Mo, Sohum Mehta, Jin Zhang
Super-resolution
activity imaging maps the biochemical architecture
of living cells yet currently overlooks the locations of collaborating
regulators/effectors. Building on the fluorescence fluctuation increase
by contact (FLINC) principle, here we devise Dronpa-chromophore-removed
FLINC (DrFLINC), where the nonfluorescent Dronpa can nevertheless
enhance TagRFP-T fluorescence fluctuations. Exploiting DrFLINC, we
develop a superior red label and a next-generation activity sensor
for context-rich super-resolution biosensing.