ac0c00037_si_001.pdf (1.29 MB)
Double-Sided Microwells with a Stepped Through-Hole Membrane for High-Throughput Microbial Assays
journal contribution
posted on 2020-07-03, 01:03 authored by Juyeol Bae, Janghyun Ju, Dahyun Kim, Taesung KimTo
improve the throughput of microwell arrays for identifying immense
cellular diversities even at a single-bacteria level, further miniaturization
or densification of the microwells has been an obvious breakthrough.
However, controlling millions of nanoliter samples or more at the
microscale remains technologically difficult and has been spatially
restricted to a single open side of the microwells. Here we employed
a stepped through-hole membrane to utilize the bottom as well as top
side of a high-density nanoliter microwell array, thus improving spatial
efficiency. The stepped structure shows additional effectiveness for
handling several millions of nanoliter bacterial samples in the overall
perspectives of controllability, throughput, simplicity, versatility,
and automation by using novel methods for three representative procedures
in bacterial assays: partitioning cells, manipulating the chemical
environment, and extracting selected cells. As a potential application,
we show proof-of-concept isolation of rare cells in a mixed ratio
of 1 to around 106 using a single chip. Our device can
be further applied to various biological studies pertaining to synthetic
biology, drug screening, mutagenesis, and single-cell heterogeneity.
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Stepped Through-Hole Membranemicrowell arrays10 6nanoliter samplesrepresentative proceduresHigh-Throughput Microbial Assaysnanoliter microwell arraychemical environmentmillionsingle-bacteria levelpartitioning cellsnovel methodsthrough-hole membraneDouble-Sided Microwellsdrug screeningthroughputshow proof-of-concept isolation
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