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Diverse Antimicrobial Killing by Enterococcus faecium E 50-52 Bacteriocin

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journal contribution
posted on 26.03.2008, 00:00 by Edward A. Svetoch, Boris V. Eruslanov, Vladimir V. Perelygin, Evgeni V. Mitsevich, Irina P. Mitsevich, Valery N. Borzenkov, Vladimir P. Levchuk, Olga E. Svetoch, Yuri N. Kovalev, Yuri G. Stepanshin, Gregory R. Siragusa, Bruce S. Seal, Norman J. Stern
An effective bacteriocin was identified and characterized. Lactic acid bacteria were screened against Campylobacter jejuni. One bacteriocin producer, Enterococcus faecium (NRRL B-30746), was studied. The isolate was grown, and the bacteriocin was purified to single-band homogeneity. Biochemical traits indicated that the peptide was a Class IIa bacteriocin, and it was named E 50-52. The bacteriocin had a molecular weight of 3339.7 and an isoelectric point of 8.0. The minimal inhibitory concentrations of E 50-52 against C. jejuni, Yersinia spp., Salmonella spp., Escherichia coli O157:H7, Shigella dysenteriae, Morganella morganii, Staphylococcus spp., and Listeria spp. ranged from 0.025 to 32 µg/mL. In therapeutic broiler trials, oral treatment with E 50-52 reduced both C. jejuni and Salmonella enteritidis by more than 100 000-fold in the ceca, and systemic S. enteritidis was reduced in the liver and spleen. The wide range of antibacterial activity of bacteriocin E 50-52 against pathogens provides a promising alternative to antibiotics.