ac050500g_si_001.pdf (47.33 kB)
Disposable Hydrophobic Surface on MALDI Targets for Enhancing MS and MS/MS Data of Peptides
journal contribution
posted on 2005-10-15, 00:00 authored by Rebekah L. Gundry, Roy Edward, Thomas P. Kole, Chris Sutton, Robert J. CotterAutomated analyses in MALDI MS are complicated by the
uneven distribution of analyte over the sample spot,
resulting in areas of analyte localization, or “sweet spots”.
Hence, the ability to concentrate and localize samples is
advantageous, especially for automated studies involving
low concentrations of analyte. A method for rapidly
creating a removable and affordable hydrophobic surface
that is free from memory effect is presented. The potential
for such compounds to serve as a practical coating for
MALDI targets is examined. An example compound with
a complete methodology is shown to increase sample
homogeneity, peak intensity, and resolution when used
for peptide mixtures with CHCA and DHB.