posted on 2022-12-19, 15:34authored byRuijie Huang, Xiaolu Meng, Kaixiang Tao, Mingming Cao, Lishuang Nie, Yao Dong, Yunbin Lyu, Shaochen Wang, Zhiyang Feng
A type II polyketide synthase biosynthetic gene cluster
(amd) containing three P450 genes was identified
from a soil
metagenomic library, and novel benz[h]isoquinoline-desferrioxamine
B conjugated compound amodesmycins were isolated from Streptomyces
albus J1074 harboring the amd gene cluster.
Genetic evidence showed that the benz[h]isoquinoline
part and desferrioxamine B part in amodesmycins were derived from
the amd gene cluster and S. albus J1074, respectively, while P450 enzymes played critical roles in
the conjunction of these two parts.