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Download fileDirect and Real-Time Observation of Rotary Movement of a DNA Nanomechanical Device
journal contribution
posted on 2013-01-23, 00:00 authored by Arivazhagan Rajendran, Masayuki Endo, Kumi Hidaka, Hiroshi SugiyamaAnalogous to the biologically abundant protein-based
linear molecular
machines that translocate along their target surface, we have recently
constructed the DNA-based synthetic molecular motors that effect linear
movement or navigate a network of tracks on a DNA origami substrate.
However, a DNA-based molecular machine with rotary function, analogous
to rotary proteins, is still unexplored. Here, we report the construction
of a rotary motor based on the B–Z conformational transition
of DNA and the direct and real-time observation of its function within
a frame-shaped DNA origami. The motor can be switched off by introducing
conditions that stabilize B-DNA, while it can be fueled by adding
Z-DNA-promoting high-saline buffer. When MgCl2 was used
as external stimulus, 70% of the motors rotated, while 76% of the
stators/controls exhibited no rotation. Such a motor system could
be successfully applied to perform multiple actions aimed for our
benefit. Moreover, for the first time we have directly observed the
B–Z conformational transition of DNA in real-time, which shed
light on the fundamental understanding of DNA conformations.