Direct and Indirect Photochemical Reactions in Viral RNA Measured with RT-qPCR and Mass Spectrometry
journal contributionposted on 07.11.2016, 00:00 authored by Zhong Qiao, Krista R. Wigginton
RNA carries the genetic instructions for many viruses to replicate in their host cells. The photochemical reactions that take place in RNA and affect viral infectivity in natural and engineered environments, however, remain poorly understood. We exposed RNA oligomer segments from the genome of bacteriophage MS2 to UV254, simulated sunlight, and singlet oxygen (1O2) and analyzed the oligomer reaction kinetics with reverse transcription quantitative PCR (RT-qPCR) and quantitative matrix-assisted laser desorption–ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Following UV254 exposure, quantitative MALDI-TOF-MS detected significantly more RNA modifications than did RT-qPCR, suggesting that certain chemical modifications in the RNA were not detected by the reverse transcriptase enzyme. In contrast, MALDI-TOF-MS tracked as much 1O2-induced RNA damage as RT-qPCR. After 5 h of simulated sunlight exposure (5100 J/m2 UVB and 1.2 × 105 J/m2 UVA), neither MALDI-TOF-MS nor RT-qPCR detected significant decreases in the oligomer concentrations. High-resolution electrospray ionization (ESI)–Orbitrap MS analyses identified pyrimidine photohydrates as the major UV254 products, which likely contributed to the discrepancy between the MS- and RT-qPCR-based results. Reactions between RNA oligomers and 1O2 resulted in an unidentified major product with a mass change of +6 Da. These results shed light on the photochemical reactions that take place in RNA and suggest that the analytical techniques used to detect RNA reactivity could bias the observed reaction kinetics.
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pyrimidine photohydratesUV 254 exposureHigh-resolution electrospray ionizationreaction kineticsUV 254 productsmass changeRNA damagemass spectrometryUVAUVBMALDI-TOFRNA oligomer segmentsMALDI-TOF-MSbacteriophage MS 2chemical modificationstranscriptase enzymeoligomer concentrationsViral RNAMass Spectrometry RNARNA oligomersIndirect Photochemical Reactions1 O 25 hRNA reactivitysinglet oxygenhost cellsRT-qPCR-based resultsUV 254RNA modificationsESIoligomer reaction kinetics