nn1c06488_si_001.pdf (1.43 MB)
Direct Nanopore Sequencing of Individual Full Length tRNA Strands
journal contributionposted on 2021-10-07, 17:39 authored by Niki K. Thomas, Vinay C. Poodari, Miten Jain, Hugh E. Olsen, Mark Akeson, Robin L. Abu-Shumays
We describe a method for direct tRNA sequencing using the Oxford Nanopore MinION. The principal technical advance is custom adapters that facilitate end-to-end sequencing of individual transfer RNA (tRNA) molecules at subnanometer precision. A second advance is a nanopore sequencing pipeline optimized for tRNA. We tested this method using purified E. coli tRNAfMet, tRNALys, and tRNAPhe samples. 76–92% of individual aligned tRNA sequence reads were full length. As a proof of concept, we showed that nanopore sequencing detected all 43 expected isoacceptors in total E. coli MRE600 tRNA as well as isodecoders that further define that tRNA population. Alignment-based comparisons between the three purified tRNAs and their synthetic controls revealed systematic nucleotide miscalls that were diagnostic of known modifications. Systematic miscalls were also observed proximal to known modifications in total E. coli tRNA alignments, including a highly conserved pseudouridine in the T loop. This work highlights the potential of nanopore direct tRNA sequencing as well as improvements needed to implement tRNA sequencing for human healthcare applications.
three purified trnasoxford nanopore minionindividual transfer rnahuman healthcare applicationshighly conserved pseudouridinealso observed proximal76 – 9243 expected isoacceptorsnanopore sequencing detecteddirect nanopore sequencingprincipal technical advancemethod using purifiedimplement trna sequencingphe supend sequencingsecond advancecoli work highlightstrna populationtrna alignmentssystematic miscallssubnanometer precisionmre600 trnaknown modificationsimprovements neededfull lengthfacilitate endcustom adaptersbased comparisons